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    Shikhar Biotech in the fight against Covid-19

    Shikhar Biotech has developed covid-19 antibody test kit. We are in the process of obtaining approval from the National Health Research Council of Nepal to test the kit on clinical samples. We are also able to determine the titre(level) of anti-covid19 antibodies in the blood of individuals who have recovered from the Covid-19 infection. This … Read more

    Flow Cytometry Training

    Shikhar Biotech has launched a free hands on training on flow cytometry to students in the final year of biotechnology courses. The training will incorporate isolation of peripheral blood monocytes (PBMCs) from blood and using them in flow cytometry to test for expression of one or more proteins. Some editions of the course will also … Read more

    Immunofluorescence of suspension cells

    Researchers try to avoid immunofluorescence on suspension cells, but don’t need to , thanks to the  Shi-fix™ cover-slips from Shikhar Biotech Immunofluorescence (IF) is a beautiful technique. Life scientists often use it to detect specific protein expression. The results are visually stunning, colorful images that show the localization of a particular protein within a cell. … Read more

    One Experiment – Two Analyses

    Combine Immunoflurescence and flow cytometry

    Combine Immunofluorescence and flow cytometry Analysis of protein expression remains a central procedure in life science research. Detection of protein expression with antibodies can use a variety of platforms like ELISA, Western Blotting, Immunofluorescence, Flow cytometry and Immunohistochemistry. Biologists are used to testing in one of these platforms at a time. This is because each … Read more

    Cell culture

    We have a wide range of cells lines under culture. Cell lysates prepared from these cells lines are routinely being used to validate our  as well as  our customer’s antibodies. We offer high quality lysates from these cell lines as well as specific tissues of your choice. If you are interested to contribute a new line, … Read more