Antibody Validation

Validated Antibodies Generate Valid Data

Reliable science starts with reliable reagents. An antibody that has not been properly validated can produce misleading results, wasting valuable time and resources. Antibody validation confirms that an antibody performs as expected in its intended application, giving researchers confidence in the accuracy, reproducibility, and reliability of their data.

Antibody Validation Service with Shikhar Biotech

We specialize in reproducible, high-quality antibody validation using western blotting, fluorescence microscopy, flow cytometry, and immunohistochemistry. We have successfully supported biotechnology companies across Europe and North America with independent antibody testing and validation projects.

Whether you need a single antibody evaluated or a large-scale validation program, we can tailor our services to meet your requirements. Simply provide your list of the antibodies, and our team will design and execute the validation workflow. All projects are handled with strict confidentiality, and we deliver reliable, datasheet-quality results at a competitive cost.

Testing with overexpressed recombinant target antigen

When the target protein is difficult to obtain or when the highest level of specificity is required, we can perform validation using cells engineered to overexpress the target antigen. This approach provides a powerful means of confirming target-specific binding and can significantly strengthen the evidence supporting antibody performance.

Why Choose Shikhar Biotech ?

Generating high-quality antibody validation data requires skilled personnel, specialized instrumentation, appropriate controls, and significant laboratory time. For many antibody manufacturers, maintaining these resources in-house can create bottlenecks and divert valuable scientific staff away from core product development activities.

Shikhar Biotech provides a flexible, scalable solution for antibody validation projects of any size. Our experienced team can rapidly design and execute validation studies across multiple applications.

By partnering with Shikhar Biotech, clients gain access to independent validation expertise without the overhead of recruiting, training, and managing additional laboratory personnel or expanding internal testing capacity.

Why customers choose Shikhar Biotech:

  • Independent, high-quality antibody validation by experienced scientists
  • Expertise in Western blotting, immunofluorescence, flow cytometry, and immunohistochemistry
  • Validation using endogenous expression systems or overexpressed recombinant targets
  • Flexible project sizes, from single antibodies to large validation programs
  • Strict confidentiality and secure handling of proprietary materials
  • Reduced pressure on internal R&D and technical support teams
  • Additional testing capacity without additional laboratory overhead
  • Reliable, datasheet-quality results delivered to support product commercialization
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We currently offer antibody-testing services through:

Michael Fiebig
Absolute Antibody

"We’ve been using Shikhar Biotech for antibody validation for over a year now and have tested around 200 antibodies in 2-3 methods with them. Their access to diverse tissues from different species has meant they have been able to test almost every clone I asked for.

The ability to choose between different testing methods and sample preparations that are ideally suited to the target antigen has made this process easy. In particular, am I fan of their immunofluorescence images, but it’s also worth noting that if your antibody doesn’t work (and you always come across a few), the team at Shikhar will tell you!"

 

How it works:

  1. You send us a list of products you want tested. If you want them tested on specific tissues or cell samples you let us know. Otherwise we will choose the most appropriate samples, and iterate as the testing progresses.
  2. We send back information on which applications we can test them on, the rate of the testing or the cost of the project, and the estimated time for the project.
  3. Once there is agreement on terms, you ship the antibodies to us, and off we go !
  4. We send you updates on the tests and compiled lots of testing results as they come.
  5. We invoice you on completion of the project.
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