Combine Immunofluorescence and flow cytometry Analysis of protein expression remains a central procedure in life science research. Detection of protein expression with antibodies can use a variety of platforms like ELISA, Western Blotting, Immunofluorescence, Flow cytometry and Immunohistochemistry. Biologists are used to testing in one of these platforms at a time. This is because each [...]
Flow Cytometry Training
Shikhar Biotech has launched a free hands on training on flow cytometry to students in the final year of biotechnology courses. The training will incorporate isolation of peripheral blood monocytes (PBMCs) from blood and using them in flow cytometry to test for expression of one or more proteins. Some editions of the course will also [...]
Immunofluorescence of suspension cells
Suspension cells are avoided in the immunofluorescence platform, but don't need to be, thanks to the Shi-fix™ cover-slips from Shikhar Biotech Immunofluorescence (IF) is a beautiful technique. Life scientists often use it to detect specific protein expression. Its beauty lies as much in the visually stunning, colorful images as in its ability to display the localization of [...]
Cell culture
We have a wide range of cells lines under culture. Cell lysates prepared from these cells lines are routinely being used to validate our as well as our customer's antibodies. We offer high quality lysates from these cell lines as well as specific tissues of your choice. If you are interested to contribute a new [...]