SHI-FIX ™ COVERSLIPS  for suspension cell Immunofluorescence

Do you wish suspension cell Immunofluorescence was as easy as of adherent cells ? Now it is! Our innovative Shi-fix™ cover-slips or multi well plates allow  suspension cells to be layered on them or directly grown  as a monolayer. Easy, hassle-free protocol and no  spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining ! Or  continue the culture to obtain desired cell densities for suspension cell immunofluorescence.

These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also  stimulate the cells while they are attached on the cover-slip, should you need to do so.

For cells that are specially difficult to attach, we have developed the PLUS line of shi-fix coverslips.

You can work on non-adherent cells as if they were adherent !

  Resuspend cultured cells in PBS

 

 

 

  Add 0.2 million cells per coverslip

 

  Wash with PBS to remove unbound cells

  Proceed to Immunofluorescence

 

                             OR

  Continue culture of attached cells 

Jurkat cell immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of Jurkat cells immobilized on SHI-FIX™ coverslips

U937 cell immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of U937 cells immobilized on SHI-FIX™ coverslips

K562 cell immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of K562 cells immobilized on SHI-FIX™ coverslips

Mouse splenocytes immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of mouse splenocytes immobilized on SHI-FIX™ coverslips

Mouse thymocytes immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of mouse thymocytes immobilized onSHI-FIX™ coverslips

Rat splenocytes immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of rat splenocytes immobilized on SHI-FIX™ coverslips

Rat thymocytes immunofluorescence on Shi-fix™ coverslips

Immunofluorescence analysis of rat thymocytes immobilized on SHI-FIX™ coverslips

Immunofluorescence of human PBMCs immobilized on Shi-fix™ coverslips

Immunofluorescence analysis of human PBMCs immobilized on SHI-FIX™ coverslips

Immunofluorescence of human PBMCs immobilized on Shi-fix™ coverslips

Immunofluorescence analysis of human PBMCs immobilized on SHI-FIX™ coverslips. The cells were also stimulated with PMA/ionomycin for 4 hours prior to fixation and permeabilization.

Immunofluorescence of human PBMCs immobilized on Shi-fix™ coverslips

Immunofluorescence analysis of Daudi cells immobilized on SHI-FIX™ coverslips. 

Immunofluorescence of human PBMCs immobilized on Shi-fix™ coverslips

Immunofluorescence analysis of Molt4 cells immobilized on SHI-FIX™ coverslips.

Publications that have used SHI-FIX™ cover-slips  successfully:

  1. Coming in and Finding Out: Blending Receptor-Targeted Delivery and Efficient Endosomal Escape in a Novel Bio-Responsive siRNA Delivery System for Gene Knockdown in Pulmonary T Cells. Kandil R, Xie Y, Heermann R, et al. Adv Ther (Weinh). 2019;2(7):1900047. doi:10.1002/adtp.201900047
  2. Specific Targeting of Somatostatin Receptor Subtype-2 for Fluorescence-Guided Surgery Servando Hernandez Vargas et al.

Prices and ordering

Product code Packing Dimensions     Price
SB-Shifix25 Pack of 25 ø 15mm x 0.17mm(#1.5) $90.00
SB-Shifix50 Pack of 50 ø 15mm x 0.17mm(#1.5) $170.00
SB-Shifix50PLUS Pack of 50 ø 15mm x 0.17mm(#1.5) $220.00
SB-Shifix96 Pack of  3 96 well plate $300.00
SB-Shifix96PLUS Pack of  3 96 well plate $400.00
  • Prices are for direct orders from the company. Prices through distributors may differ.
  • The cost of shipping worldwide by Fedex  is USD100. The product is normally delivered to your lab by FEDEX unless there are special import restrictions in your country- in that case you may need to contact the customs office of your country.
  • The HS code of the product is 70179090
  • Once you let us know the exact quantity you wish to order, we can send a proforma invoice for payment to our bank. Goods are dispatched from our lab within 3 working days after payment arrives in our bank. We can not process payments by credit cards.